Influence of Beta Tricalcium Phosphate Particles on In Vitro Material Degradation and Early Cell Attachment of MC3T3-E1 Cells on Poly(3-Hydroxybutyrate)/Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate) Films
Kopf, Sabrina; Kylmaoja, Elina; Akesson, Dan; Persson, Maria; Skrifvars, Mikael (2025-07-04)
John Wiley & Sons
04.07.2025
Kopf, S., Kylmäoja, E., Åkesson, D., Persson, M., & Skrifvars, M. (2025). Influence of beta tricalcium phosphate particles on in vitro material degradation and early cell attachment of MC3T3-E1 cells on poly(3‐hydroxybutyrate)/poly(3‐hydroxybutyrate‐co‐4‐hydroxybutyrate) films. Journal of Applied Polymer Science, 142(40), e57549. https://doi.org/10.1002/app.57549
https://creativecommons.org/licenses/by/4.0/
© 2025 The Author(s). Journal of Applied Polymer Science published by Wiley Periodicals LLC. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium,provided the original work is properly cited.
https://creativecommons.org/licenses/by/4.0/
© 2025 The Author(s). Journal of Applied Polymer Science published by Wiley Periodicals LLC. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium,provided the original work is properly cited.
https://creativecommons.org/licenses/by/4.0/
Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi:oulu-202508155354
https://urn.fi/URN:NBN:fi:oulu-202508155354
Tiivistelmä
Abstract
The influence of beta-tricalcium phosphate (ß-TCP) particles on the early cell attachment of MC3T3-E1 osteoblast precursor cells is investigated on a poly(3-hydroxybutyrate)/poly(3-hydroxybutyrate-co-4-hydroxybutyrate) polymer blend. MC3T3-E1 cells adhere to both polymer blends, with and without ß-TCP. In both cases, the cells show a typical cell morphology and focal adhesions on the polymer surface. The ß-TCP does not significantly improve the cell adhesion and hydrophilicity of the polymer films. Further, ß-TCP does not alter the degradation behavior of the poly(3-hydroxybutyrate)/poly(3-hydroxybutyrate-co-4-hydroxybutyrate) blend when exposed to phosphate buffered saline solution for up to 70 days. However, the results confirm that the polyhydroxyalkanoate blends used in this study are non-cytotoxic and maintain structural integrity over time. These findings highlight the blend's promise for usage in long-term biomedical applications, particularly in bone tissue engineering, because of their stability in isotonic medium.
The influence of beta-tricalcium phosphate (ß-TCP) particles on the early cell attachment of MC3T3-E1 osteoblast precursor cells is investigated on a poly(3-hydroxybutyrate)/poly(3-hydroxybutyrate-co-4-hydroxybutyrate) polymer blend. MC3T3-E1 cells adhere to both polymer blends, with and without ß-TCP. In both cases, the cells show a typical cell morphology and focal adhesions on the polymer surface. The ß-TCP does not significantly improve the cell adhesion and hydrophilicity of the polymer films. Further, ß-TCP does not alter the degradation behavior of the poly(3-hydroxybutyrate)/poly(3-hydroxybutyrate-co-4-hydroxybutyrate) blend when exposed to phosphate buffered saline solution for up to 70 days. However, the results confirm that the polyhydroxyalkanoate blends used in this study are non-cytotoxic and maintain structural integrity over time. These findings highlight the blend's promise for usage in long-term biomedical applications, particularly in bone tissue engineering, because of their stability in isotonic medium.
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