Gut microbiota-derived extracellular vesicles form a distinct entity from gut microbiota
Kaisanlahti, Anna; Turunen, Jenni; Hekkala, Jenni; Mishra, Surbhi; Karikka, Sonja; Amatya, Sajeen Bahadur; Paalanne, Niko; Kruger, Johanna; Portaankorva, Anne M; Koivunen, Jussi; Jukkola, Arja; Vihinen, Pia; Auvinen, Päivi; Leppä, Sirpa; Karihtala, Peeter; Koivukangas, Vesa; Hukkanen, Janne; Vainio, Seppo; Samoylenko, Anatoliy; Bart, Genevieve; Lahti, Leo; Reunanen, Justus; Tejesvi, Mysore V; Ruuska-Loewald, Terhi (2025-04-29)
American Society for Microbiology
29.04.2025
Kaisanlahti A, Turunen J, Hekkala J, Mishra S, Karikka S, Amatya SB, Paalanne N, Kruger J, Portaankorva AM, Koivunen J, Jukkola A, Vihinen P, Auvinen P, Leppä S, Karihtala P, Koivukangas V, Hukkanen J, Vainio S, Samoylenko A, Bart G, Lahti L, Reunanen J, Tejesvi MV, Ruuska-Loewald T. 2025. Gut microbiota-derived extracellular vesicles form a distinct entity from gut microbiota. mSystems10:e00311-25. https://doi.org/10.1128/msystems.00311-25
https://creativecommons.org/licenses/by/4.0/
Copyright © 2025 Kaisanlahti et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
https://creativecommons.org/licenses/by/4.0/
Copyright © 2025 Kaisanlahti et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
https://creativecommons.org/licenses/by/4.0/
Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi:oulu-202504303008
https://urn.fi/URN:NBN:fi:oulu-202504303008
Tiivistelmä
Abstract
Extracellular vesicles (EVs), nanoparticles secreted by both gram-negative and gram-positive bacteria, carry various biomolecules and cross biological barriers. Gut microbiota-derived EVs are currently being investigated as a communication mechanism between the microbiota and the host. Few clinical studies, however, have investigated gut microbiota-derived EVs. Here, we show that machine learning models were able to accurately distinguish gut microbiota and respective microbiota-derived EV samples according to their taxonomic composition both within each data set (area under the curve [AUC] 0.764–1.00) and in a cross-study setting (AUC 0.701–0.997). These results show that gut microbiota-derived EVs form a distinct taxonomic entity from gut microbiota. Thus, conventional gut microbiota composition may not correctly reflect communication between the gut microbiota and the host unless microbiota-derived EVs are reported separately.
Extracellular vesicles (EVs), nanoparticles secreted by both gram-negative and gram-positive bacteria, carry various biomolecules and cross biological barriers. Gut microbiota-derived EVs are currently being investigated as a communication mechanism between the microbiota and the host. Few clinical studies, however, have investigated gut microbiota-derived EVs. Here, we show that machine learning models were able to accurately distinguish gut microbiota and respective microbiota-derived EV samples according to their taxonomic composition both within each data set (area under the curve [AUC] 0.764–1.00) and in a cross-study setting (AUC 0.701–0.997). These results show that gut microbiota-derived EVs form a distinct taxonomic entity from gut microbiota. Thus, conventional gut microbiota composition may not correctly reflect communication between the gut microbiota and the host unless microbiota-derived EVs are reported separately.
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Ellei muuten mainita, aineiston lisenssi on https://creativecommons.org/licenses/by/4.0/