Scanning Transmission Soft X-ray Microscopy of Cell and Tissue Samples from Spruces, Mice and Humans
Mansikkala, Tuomas (2025-04-23)
© University of Oulu, 2024. This publication is copyrighted. You may download, display and print it for your own personal use. Commercial use is prohibited. © Oulun yliopisto, 2024. Julkaisu on tekijänoikeussäännösten alainen. Teosta voi lukea ja tulostaa henkilökohtaista käyttöä varten. Käyttö kaupallisiin tarkoituksiin on kielletty.
https://rightsstatements.org/vocab/InC/1.0/
https://urn.fi/URN:NBN:fi:oulu-202504232835
Kuvaus
Tiivistelmä
This thesis covers studies of tissue and cell samples with scanning transmission X-ray spectroscopy (STXM) in soft X-ray energy range. The focus was on finding out how well accumulated biomaterials can be detected and measured in biological specimens. The plant study was done in collaboration with Natural Resources Institute of Finland (Luke) to detect and differentiate valuable lignans from structural biomolecules in Norway spruce knotwood. We were able to detect lignans in the cell lumen and differentiate them from lignins which are part of the cell wall. The experiments on mouse and human cells and tissues were done in collaboration with Pediatric Neurology Research Group, University of Oulu, Finland, to study sialic acid accumulation in Salla disease. The study was done with genetically modified Slc17a5 knockout mouse tissues and with Salla disease patient-derived fibroblasts. We were unable to detect the sialic acid accumulations in the Salla disease experiments, but this seemed to be a problem caused by common sample preparation methods. We were, however, able to distinguish cellular compartments using STXM. The overall result was that STXM can differentiate biological materials within a cell, however common sample preparation methods seem to remove water-soluble materials, leaving mostly structural materials intact. With further sample preparation development aiming to retain soluble materials, STXM should be a good tool to study cell and tissue samples.
Original papers
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Mansikkala, T., Patanen, M., Kärkönen, A., Korpinen, R., Pranovich, A., Ohigashi, T., Swaraj, S., Seitsonen, J., Ruokolainen, J., Huttula, M., Saranpää, P., & Piispanen, R. (2020). Lignans in knotwood of norway spruce: Localisation with soft x-ray microscopy and scanning transmission electron microscopy with energy dispersive x-ray spectroscopy. Molecules, 25(13), 2997. https://doi.org/10.3390/molecules25132997. https://doi.org/10.3390/molecules25132997
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Mansikkala, T., Ohigashi, T., Salo, M. H., Hiltunen, A. E., Vuolteenaho, R., Sipilä, P., Kuure, S., Huttula, M., Uusimaa, J., Hinttala, R., Miinalainen, I., Kangas, S., & Patanen, M. (2023). Scanning transmission soft X-ray spectromicroscopy of mouse kidney and liver. Journal of Electron Spectroscopy and Related Phenomena, 266, 147368. https://doi.org/10.1016/j.elspec.2023.147368. https://doi.org/10.1016/j.elspec.2023.147368
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Mansikkala, T., Kangas, S. M., Miinalainen, I., Angervaniva, P., Darin, N., Blomqvist, M., Hinttala, R., Huttula, M., Uusimaa, J., & Patanen, M. (2024). Soft X-ray spectromicroscopy of human fibroblasts with impaired sialin function. RSC Advances, 14(39), 28797–28806. https://doi.org/10.1039/D4RA05520A. https://doi.org/10.1039/D4RA05520A
Osajulkaisut
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Mansikkala, T., Patanen, M., Kärkönen, A., Korpinen, R., Pranovich, A., Ohigashi, T., Swaraj, S., Seitsonen, J., Ruokolainen, J., Huttula, M., Saranpää, P., & Piispanen, R. (2020). Lignans in knotwood of norway spruce: Localisation with soft x-ray microscopy and scanning transmission electron microscopy with energy dispersive x-ray spectroscopy. Molecules, 25(13), 2997. https://doi.org/10.3390/molecules25132997. https://doi.org/10.3390/molecules25132997
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Mansikkala, T., Ohigashi, T., Salo, M. H., Hiltunen, A. E., Vuolteenaho, R., Sipilä, P., Kuure, S., Huttula, M., Uusimaa, J., Hinttala, R., Miinalainen, I., Kangas, S., & Patanen, M. (2023). Scanning transmission soft X-ray spectromicroscopy of mouse kidney and liver. Journal of Electron Spectroscopy and Related Phenomena, 266, 147368. https://doi.org/10.1016/j.elspec.2023.147368. https://doi.org/10.1016/j.elspec.2023.147368
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Mansikkala, T., Kangas, S. M., Miinalainen, I., Angervaniva, P., Darin, N., Blomqvist, M., Hinttala, R., Huttula, M., Uusimaa, J., & Patanen, M. (2024). Soft X-ray spectromicroscopy of human fibroblasts with impaired sialin function. RSC Advances, 14(39), 28797–28806. https://doi.org/10.1039/D4RA05520A. https://doi.org/10.1039/D4RA05520A
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