Isolation and phenotypic characterization of cancer stem cells from metastatic oral cancer cells
Aquino, Iara Gonçalves; Cuadra-Zelaya, Florence Juana Maria; Bizeli, Ana Laura Valença; Palma, Patricia Vianna Bonini; Mariano, Fernanda Viviane; Salo, Tuula; Coletta, Ricardo Della; Bastos, Débora Campanella; Graner, Edgard (2024-05-20)
Wiley-Blackwell
20.05.2024
Aquino, I. G., Cuadra-Zelaya, F. J. M., Bizeli, A. L. V., Palma, P. V. B., Mariano, F. V., Salo, T., Coletta, R. D., Bastos, D. C., & Graner, E. (2024). Isolation and phenotypic characterization of cancer stem cells from metastatic oral cancer cells. Oral Diseases, 30, 4886–4897. https://doi.org/10.1111/odi.15003
https://rightsstatements.org/vocab/InC/1.0/
© 2024 Wiley Periodicals LLC. This is the peer reviewed version of the following article: Aquino, I. G., Cuadra-Zelaya, F. J. M., Bizeli, A. L. V., Palma, P. V. B., Mariano, F. V., Salo, T., Coletta, R. D., Bastos, D. C., & Graner, E. (2024). Isolation and phenotypic characterization of cancer stem cells from metastatic oral cancer cells. Oral Diseases, 00, 1–12, which has been published in final form at https://doi.org/10.1111/odi.15003. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
https://rightsstatements.org/vocab/InC/1.0/
© 2024 Wiley Periodicals LLC. This is the peer reviewed version of the following article: Aquino, I. G., Cuadra-Zelaya, F. J. M., Bizeli, A. L. V., Palma, P. V. B., Mariano, F. V., Salo, T., Coletta, R. D., Bastos, D. C., & Graner, E. (2024). Isolation and phenotypic characterization of cancer stem cells from metastatic oral cancer cells. Oral Diseases, 00, 1–12, which has been published in final form at https://doi.org/10.1111/odi.15003. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
https://rightsstatements.org/vocab/InC/1.0/
Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi:oulu-202406274970
https://urn.fi/URN:NBN:fi:oulu-202406274970
Tiivistelmä
Abstract
Objectives:
To isolate cancer stem cells (CSC) from a metastatic oral squamous cell carcinoma (OSCC) cell line and investigate their in vitro and in vivo phenotypic characteristics.
Materials and Methods:
Subpopulations with individual staining intensities for CD44 and CD326 were isolated from the OSCC cell line LN-1A by FACS: CD44Low/CD326− (CSC-M1), CD44Low/CD326High (CSC-E), and CD44High/CD326− (CSC-M2). Proliferation, clonogenic potential, adhesion, migration, epithelial-mesenchymal transition markers, and sensitivity to cisplatin and TVB-3166 were analyzed in vitro. Tumor formation and metastasis were assessed by subcutaneous and orthotopic inoculations into BALB/c mice.
Results:
E-cadherin levels were higher in CSC-E cells while vimentin and Slug more produced by CSC-M2 cells. CSC-M1 and CSC-M2 subpopulations showed higher proliferation, produced more colonies, and have stronger adhesion to the extracellular matrix. All cell lines established tumors; however, CSC-E and CSC-M2 formed larger masses and produced more metastases.
Conclusion:
The CSC subpopulations here described show increased cancer capabilities in vitro, tumorigenic and metastatic potential in vivo, and may be exploited in the search for novel therapeutic targets for OSCC.
Objectives:
To isolate cancer stem cells (CSC) from a metastatic oral squamous cell carcinoma (OSCC) cell line and investigate their in vitro and in vivo phenotypic characteristics.
Materials and Methods:
Subpopulations with individual staining intensities for CD44 and CD326 were isolated from the OSCC cell line LN-1A by FACS: CD44Low/CD326− (CSC-M1), CD44Low/CD326High (CSC-E), and CD44High/CD326− (CSC-M2). Proliferation, clonogenic potential, adhesion, migration, epithelial-mesenchymal transition markers, and sensitivity to cisplatin and TVB-3166 were analyzed in vitro. Tumor formation and metastasis were assessed by subcutaneous and orthotopic inoculations into BALB/c mice.
Results:
E-cadherin levels were higher in CSC-E cells while vimentin and Slug more produced by CSC-M2 cells. CSC-M1 and CSC-M2 subpopulations showed higher proliferation, produced more colonies, and have stronger adhesion to the extracellular matrix. All cell lines established tumors; however, CSC-E and CSC-M2 formed larger masses and produced more metastases.
Conclusion:
The CSC subpopulations here described show increased cancer capabilities in vitro, tumorigenic and metastatic potential in vivo, and may be exploited in the search for novel therapeutic targets for OSCC.
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