Serum reactivity in dermatitis herpetiformis: an international multicentre study
Boch, Katharina; Heck, Florian; Hammers, Christoph M; Antiga, Emiliano; Caproni, Marzia; Juhl, David; Goletz, Stephanie; Horváth, Orsolya N; Huilaja, Laura; Khil’chenko, Stanislav; Sina, Christian; Tasanen, Kaisa; Vassileva, Snejina; Schlumberger, Wolfgang; Zillikens, Detlef; Shahid, Martin; Drenovska, Kossara; Zone, John J; Koszorú, Kamilla; Fechner, Kai; Dähnrich, Cornelia; König, Inke R; Schmidt, Enno (2023-10-04)
Avaa tiedosto
Sisältö avataan julkiseksi: 04.10.2024
Oxford University Press
04.10.2023
Katharina Boch, Florian Heck, Christoph M Hammers, Emiliano Antiga, Marzia Caproni, David Juhl, Stephanie Goletz, Orsolya N Horváth, Laura Huilaja, Stanislav Khil’chenko, Christian Sina, Kaisa Tasanen, Snejina Vassileva, Wolfgang Schlumberger, Detlef Zillikens, Martin Shahid, Kossara Drenovska, John J Zone, Kamilla Koszorú, Kai Fechner, Cornelia Dähnrich, Inke R König, Enno Schmidt, Serum reactivity in dermatitis herpetiformis: an international multicentre study, Clinical and Experimental Dermatology, Volume 49, Issue 1, January 2024, Pages 53–57, https://doi.org/10.1093/ced/llad319
https://rightsstatements.org/vocab/InC/1.0/
© The Author(s) 2023. Published by Oxford University Press on behalf of British Association of Dermatologists. All rights reserved. This is a pre-copyedited, author-produced version of an article accepted for publication in Clinical and Experimental Dermatology following peer review. The version of record Boch, K., Heck, F., Hammers, C. M., Antiga, E., Caproni, M., Juhl, D., Goletz, S., Horváth, O. N., Huilaja, L., Khil’chenko, S., Sina, C., Tasanen, K., Vassileva, S., Schlumberger, W., Zillikens, D., Shahid, M., Drenovska, K., Zone, J. J., Koszorú, K., … Schmidt, E. (2023). Serum reactivity in dermatitis herpetiformis: An international multicentre study. Clinical and Experimental Dermatology, 49(1), 53–57 is available online at: https://doi.org/10.1093/ced/llad319
https://rightsstatements.org/vocab/InC/1.0/
© The Author(s) 2023. Published by Oxford University Press on behalf of British Association of Dermatologists. All rights reserved. This is a pre-copyedited, author-produced version of an article accepted for publication in Clinical and Experimental Dermatology following peer review. The version of record Boch, K., Heck, F., Hammers, C. M., Antiga, E., Caproni, M., Juhl, D., Goletz, S., Horváth, O. N., Huilaja, L., Khil’chenko, S., Sina, C., Tasanen, K., Vassileva, S., Schlumberger, W., Zillikens, D., Shahid, M., Drenovska, K., Zone, J. J., Koszorú, K., … Schmidt, E. (2023). Serum reactivity in dermatitis herpetiformis: An international multicentre study. Clinical and Experimental Dermatology, 49(1), 53–57 is available online at: https://doi.org/10.1093/ced/llad319
https://rightsstatements.org/vocab/InC/1.0/
Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi:oulu-202405223825
https://urn.fi/URN:NBN:fi:oulu-202405223825
Tiivistelmä
Abstract
Background:
Dermatitis herpetiformis (DH) is a rare gluten-induced skin disorder characterized predominantly by IgA autoantibodies against endomysium, tissue transglutaminase (TG2/tTG), epidermal transglutaminase (TG3/eTG) and deamidated gliadin. To date, circulating autoantibody reactivity has not been systematically described.
Objectives:
Characterization of serum reactivities in DH.
Methods:
This multicentre international study analysed sera from 242 patients with DH taken at the time of initial diagnosis. DH-specific IgA and IgG serum autoantibodies were analysed by indirect immunofluorescence (IF) on monkey oesophagus, and by enzyme-linked immunosorbent assay (ELISA) based on recombinant TG2/tTG, TG3/eTG and deamidated gliadin (GAF3X).
Results:
IgA indirect IF microscopy on monkey oesophagus revealed the highest reactivity (84.3%; specificity 100%) followed by IgA TG2/tTG ELISA (78.5%, specificity 99.0%), IgA TG3/eTG ELISA (72.7%, specificity 95.0%) and IgA GAF3X ELISA (69.0%, specificity 98.5%).
Conclusions:
Serum IgA and IgG autoantibodies against endomysium, TG2/tTG, TG3/eTG and deamidated gliadin are highly prevalent in DH. Indirect IF microscopy on monkey oesophagus (IgA) provides the highest diagnostic accuracy that can be further enhanced by 4.5% when combined with IgA TG2/tTG ELISA.
Background:
Dermatitis herpetiformis (DH) is a rare gluten-induced skin disorder characterized predominantly by IgA autoantibodies against endomysium, tissue transglutaminase (TG2/tTG), epidermal transglutaminase (TG3/eTG) and deamidated gliadin. To date, circulating autoantibody reactivity has not been systematically described.
Objectives:
Characterization of serum reactivities in DH.
Methods:
This multicentre international study analysed sera from 242 patients with DH taken at the time of initial diagnosis. DH-specific IgA and IgG serum autoantibodies were analysed by indirect immunofluorescence (IF) on monkey oesophagus, and by enzyme-linked immunosorbent assay (ELISA) based on recombinant TG2/tTG, TG3/eTG and deamidated gliadin (GAF3X).
Results:
IgA indirect IF microscopy on monkey oesophagus revealed the highest reactivity (84.3%; specificity 100%) followed by IgA TG2/tTG ELISA (78.5%, specificity 99.0%), IgA TG3/eTG ELISA (72.7%, specificity 95.0%) and IgA GAF3X ELISA (69.0%, specificity 98.5%).
Conclusions:
Serum IgA and IgG autoantibodies against endomysium, TG2/tTG, TG3/eTG and deamidated gliadin are highly prevalent in DH. Indirect IF microscopy on monkey oesophagus (IgA) provides the highest diagnostic accuracy that can be further enhanced by 4.5% when combined with IgA TG2/tTG ELISA.
Kokoelmat
- Avoin saatavuus [34357]