Benchmarking blood collection tubes and processing intervals for extracellular vesicle performance metrics
Dhondt, Bert; Pinheiro, Cláudio; Geeurickx, Edward; Tulkens, Joeri; Vergauwen, Glenn; Van Der Pol, Edwin; Nieuwland, Rienk; Decock, Anneleen; Miinalainen, Ilkka; Rappu, Pekka; Schroth, Gary; Kuersten, Scott; Vandesompele, Jo; Mestdagh, Pieter; Lumen, Nicolaas; De Wever, Olivier; Hendrix, An (2023-05-18)
Dhondt, B., Pinheiro, C., Geeurickx, E., Tulkens, J., Vergauwen, G., Van Der Pol, E., Nieuwland, R., Decock, A., Miinalainen, I., Rappu, P., Schroth, G., Kuersten, S., Vandesompele, J., Mestdagh, P., Lumen, N., De Wever, O., & Hendrix, A. (2023). Benchmarking blood collection tubes and processing intervals for extracellular vesicle performance metrics. Journal of Extracellular Vesicles, 12, e12315. https://doi.org/10.1002/jev2.12315
© 2023 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
https://creativecommons.org/licenses/by-nc-nd/4.0/
https://urn.fi/URN:NBN:fi-fe20230906120234
Tiivistelmä
Abstract
The analysis of extracellular vesicles (EV) in blood samples is under intense investigation and holds the potential to deliver clinically meaningful biomarkers for health and disease. Technical variation must be minimized to confidently assess EV-associated biomarkers, but the impact of pre-analytics on EV characteristics in blood samples remains minimally explored. We present the results from the first large-scale EV Blood Benchmarking (EVBB) study in which we systematically compared 11 blood collection tubes (BCT; six preservation and five non-preservation) and three blood processing intervals (BPI; 1, 8 and 72 h) on defined performance metrics (n = 9). The EVBB study identifies a significant impact of multiple BCT and BPI on a diverse set of metrics reflecting blood sample quality, ex-vivo generation of blood-cell derived EV, EV recovery and EV-associated molecular signatures. The results assist the informed selection of the optimal BCT and BPI for EV analysis. The proposed metrics serve as a framework to guide future research on pre-analytics and further support methodological standardization of EV studies.
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