Infrared spectroscopy is suitable for objective assessment of articular cartilage health
Virtanen, V.; Tafintseva, V.; Shaikh, R.; Nippolainen, E.; Haas, J.; Afara, I. O.; Töyräs, J.; Kröger, H.; Solheim, J.; Zimmermann, B.; Kohler, A.; Mizaikoff, B.; Finnilä, M.; Rieppo, L.; Saarakkala, S. (2022-03-15)
V. Virtanen, V. Tafintseva, R. Shaikh, E. Nippolainen, J. Haas, I.O. Afara, J. Töyräs, H. Kröger, J. Solheim, B. Zimmermann, A. Kohler, B. Mizaikoff, M. Finnilä, L. Rieppo, S. Saarakkala, Infrared spectroscopy is suitable for objective assessment of articular cartilage health, Osteoarthritis and Cartilage Open, Volume 4, Issue 2, 2022, 100250, ISSN 2665-9131, https://doi.org/10.1016/j.ocarto.2022.100250
© 2022 The Authors. Published by Elsevier Ltd on behalf of Osteoarthritis Research Society International (OARSI). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
https://creativecommons.org/licenses/by-nc-nd/4.0/
https://urn.fi/URN:NBN:fi-fe2023062965702
Tiivistelmä
Abstract
Objective: To evaluate the feasibility of Fourier transform infrared attenuated total reflectance (FTIR-ATR) spectroscopy to detect cartilage degradation due to osteoarthritis and to validate the methodology with osteochondral human cartilage samples for future development towards clinical use.
Design: Cylindrical (d = 4 mm) osteochondral samples (n = 349) were prepared from nine human cadavers and measured with FTIR-ATR spectroscopy. Afterwards, the samples were assessed with Osteoarthritis Research Society International (OARSI) osteoarthritis cartilage histopathology assessment system and divided into two groups: 1) healthy (OARSI 0–2) and 2) osteoarthritic (OARSI 2.5–6). The classification was done with partial least squares discriminant analysis model utilizing cross-model validation. Receiver operating characteristics curve analysis was performed and the area under curve (AUC) was calculated.
Results: For all samples combined, classification accuracy was 73% with AUC of 0.79. Femoral samples had accuracy of 74% and AUC of 0.77, while tibial samples had accuracy of 66%, and AUC of 0.74. Patellar samples had accuracy of 84% and AUC of 0.91.
Conclusions: The results indicate that FTIR-ATR spectroscopy can differentiate between healthy and osteoarthritic femoral, tibial and patellar human tissue. If combined with a fiber optic probe, FTIR-ATR spectroscopy could provide additional objective intraoperative information during arthroscopic surgeries, which could improve clinical outcomes.
Kokoelmat
- Avoin saatavuus [38599]
