Ex vivo confocal Raman microspectroscopy of porcine dura mater supported by optical clearing
Jaafar, Ali; Holomb, Roman; Sdobnov, Anton Y.; Ocskay, Zsombor; Jakus, Zoltán; Tuchin, Valery V.; Veres, Miklós (2021-12-24)
Jaafar, A., Holomb, R., Sdobnov, A. Y., Ocskay, Z., Jakus, Z., Tuchin, V. V., Veres, M., J. Biophotonics 2022, 15( 4), e202100332. https://doi.org/10.1002/jbio.202100332
© 2021 Wiley-VCH GmbH. This is the peer reviewed version of the following article: Jaafar, A., Holomb, R., Sdobnov, A. Y., Ocskay, Z., Jakus, Z., Tuchin, V. V., Veres, M., J. Biophotonics 2022, 15( 4), e202100332. https://doi.org/10.1002/jbio.202100332, which has been published in final form at https://doi.org/10.1002/jbio.202100332. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
https://rightsstatements.org/vocab/InC/1.0/
https://urn.fi/URN:NBN:fi-fe2023040535156
Tiivistelmä
Abstract
The effect of tissue optical clearing (TOC) to increase the probing depth and observe in-depth structure of the ex vivo porcine dura mater was studied by confocal Raman microspectroscopy (CRM). Raman intensities were significantly increased at the depth of 250 μm for all collagen bands after treatment with glycerol. The influence of glycerol on collagen hydration was also investigated. The results indicate that the process of TOC can be divided into three main steps. The first one is a fast process of tissue dehydration accompanied by collagen shrinkage while the second relatively slow process is related to the glycerol penetration into the interfibrillar space of collagen combined with swelling of tissue. The third step is collagen dissociation caused by the high concentration of glycerol. To the best of our knowledge, this study is the first example to introduce the TOC technique in assisting CRM of ex vivo dura mater in-depth probing.
Kokoelmat
- Avoin saatavuus [38840]