Detection of cell-derived exosomes via surface-enhanced Raman scattering using aggregated silver nanoparticles
Li, Yang; Wang, Yunpeng; Tian, Jinwei; Huang, Jian-An (2023-05-05)
Li, Y., Wang, Y., Tian, J., & Huang, J.-A. (2023). Detection of cell-derived exosomes via surface-enhanced raman scattering using aggregated silver nanoparticles. In S. Vainio (Ed.), Cell-Secreted Vesicles (Vol. 2668, pp. 15–22). Springer US. https://doi.org/10.1007/978-1-0716-3203-1_2
© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. This version of the article has been accepted for publication, after peer review, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: https://doi.org/10.1007/978-1-0716-3203-1_2. Use of this Accepted Version is subject to the publisher’s Accepted Manuscript terms of use https://www.springernature.com/gp/open-research/policies/accepted-manuscript-terms”.
https://rightsstatements.org/vocab/InC/1.0/
https://urn.fi/URN:NBN:fi-fe2023051243929
Tiivistelmä
Abstract
Exosomes are small extracellular vesicles that contain RNA, lipids, and proteins and can act as cellular messengers, carrying information to cells and tissues in the body. Thus, sensitive, label-free, and multiplexed analysis of exosomes may help in early diagnosis of important diseases. Here, we describe the process of pretreatment of cell-derived exosomes, preparation of surface-enhanced Raman scattering (SERS) substrates, and label-free SERS detection of exosomes using sodium borohydride aggregators. This method can enable the observation of SERS signals of exosomes that are clear and stable and have a good signal-to-noise ratio.
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