Xenogenic neural stem cell-derived extracellular nanovesicles modulate human mesenchymal stem cell fate and reconstruct metabolomic structure
Derkus, Burak; Isik, Melis; Eylem, Cemil Can; Ergin, Irem; Camci, Can Berk; Bilgin, Sila; Elbuken, Caglar; Arslan, Yavuz Emre; Akkulak, Merve; Adali, Orhan; Kiran, Fadime; Okesola, Babatunde O.; Nemutlu, Emirhan; Emregul, Emel (2022-03-28)
Derkus, B., Isik, M., Eylem, C. C., Ergin, I., Camci, C. B., Bilgin, S., Elbuken, C., Arslan, Y. E., Akkulak, M., Adali, O., Kiran, F., Okesola, B. O., Nemutlu, E., Emregul, E., Xenogenic Neural Stem Cell-Derived Extracellular Nanovesicles Modulate Human Mesenchymal Stem Cell Fate and Reconstruct Metabolomic Structure. Adv. Biology 2022, 6, 2101317. https://doi.org/10.1002/adbi.202101317
© 2022 Wiley-VCH GmbH. This is the peer reviewed version of the following article: Derkus, B., Isik, M., Eylem, C. C., Ergin, I., Camci, C. B., Bilgin, S., Elbuken, C., Arslan, Y. E., Akkulak, M., Adali, O., Kiran, F., Okesola, B. O., Nemutlu, E., Emregul, E., Xenogenic Neural Stem Cell-Derived Extracellular Nanovesicles Modulate Human Mesenchymal Stem Cell Fate and Reconstruct Metabolomic Structure. Adv. Biology 2022, 6, 2101317, which has been published in final form at https://doi.org/10.1002/adbi.202101317. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
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https://urn.fi/URN:NBN:fi-fe202301193637
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Abstract
Extracellular nanovesicles, particularly exosomes, can deliver their diverse bioactive biomolecular content, including miRNAs, proteins, and lipids, thus providing a context for investigating the capability of exosomes to induce stem cells toward lineage-specific cells and tissue regeneration. In this study, it is demonstrated that rat subventricular zone neural stem cell-derived exosomes (rSVZ-NSCExo) can control neural-lineage specification of human mesenchymal stem cells (hMSCs). Microarray analysis shows that the miRNA content of rSVZ-NSCExo is a faithful representation of rSVZ tissue. Through immunocytochemistry, gene expression, and multi-omics analyses, the capability to use rSVZ-NSCExo to induce hMSCs into a neuroglial or neural stem cell phenotype and genotype in a temporal and dose-dependent manner via multiple signaling pathways is demonstrated. The current study presents a new and innovative strategy to modulate hMSCs fate by harnessing the molecular content of exosomes, thus suggesting future opportunities for rSVZ-NSCExo in nerve tissue regeneration.
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