A hunt for <em>OM45</em> synthetic petite interactions in <em>Saccharomyces cerevisiae</em> reveals a role for Miro GTPase Gem1p in cristae structure maintenance
Shvetsova, Antonina; Masud, Ali J.; Schneider, Laura; Bergmann, Ulrich; Monteuuis, Geoffray; Miinalainen, Ilkka J.; Hiltunen, J. Kalervo; Kastaniotis, Alexander J. (2021-10-08)
Shvetsova, A., Masud, A. J., Schneider, L., Bergmann, U., Monteuuis, G., Miinalainen, I. J., Hiltunen, J. K., & Kastaniotis, A. J. (2021). A hunt for OM45 synthetic petite interactions in Saccharomyces cerevisiae reveals a role for Miro GTPase Gem1p in cristae structure maintenance. MicrobiologyOpen, 10, e1238. https://doi.org/10.1002/mbo3.1238
© 2021 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
https://creativecommons.org/licenses/by-nc-nd/4.0/
https://urn.fi/URN:NBN:fi-fe2022013111302
Tiivistelmä
Abstract
Om45 is a major protein of the yeast’s outer mitochondrial membrane under respiratory conditions. However, the cellular role of the protein has remained obscure. Previously, deletion mutant phenotypes have not been found, and clear amino acid sequence similarities that would allow inferring its functional role are not available. In this work, we describe synthetic petite mutants of GEM1 and UGO1 that depend on the presence of OM45 for respiratory growth, as well as the identification of several multicopy suppressors of the synthetic petite phenotypes. In the analysis of our mutants, we demonstrate that Om45p and Gem1p have a collaborative role in the maintenance of mitochondrial morphology, cristae structure, and mitochondrial DNA maintenance. A group of multicopy suppressors rescuing the synthetic lethal phenotypes of the mutants on non-fermentable carbon sources additionally supports this result. Our results imply that the synthetic petite phenotypes we observed are due to the disturbance of the inner mitochondrial membrane and point to this mitochondrial sub-compartment as the main target of action of Om45p, Ugo1p, and the yeast Miro GTPase Gem1p.
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