Arc self-association and formation of virus-like capsids are mediated by an N-terminal helical coil motif
Eriksen, Maria S.; Nikolaienko, Oleksii; Hallin, Erik I.; Grødem, Sverre; Bustad, Helene J.; Flydal, Marte I.; Merski, Ian; Hosokawa, Tomohisa; Lascu, Daniela; Akerkar, Shreeram; Cuéllar, Jorge; Chambers, James J.; O’Connell, Rory; Muruganandam, Gopinath; Loris, Remy; Touma, Christine; Kanhema, Tambudzai; Hayashi, Yasunori; Stratton, Margaret M.; Valpuesta, José M.; Kursula, Petri; Martinez, Aurora; Bramham, Clive R. (2020-11-11)
Eriksen, M.S., Nikolaienko, O., Hallin, E.I., Grødem, S., Bustad, H.J., Flydal, M.I., Merski, I., Hosokawa, T., Lascu, D., Akerkar, S., Cuéllar, J., Chambers, J.J., O’Connell, R., Muruganandam, G., Loris, R., Touma, C., Kanhema, T., Hayashi, Y., Stratton, M.M., Valpuesta, J.M., Kursula, P., Martinez, A. and Bramham, C.R. (2021), Arc self-association and formation of virus-like capsids are mediated by an N-terminal helical coil motif. FEBS J, 288: 2930-2955. https://doi.org/10.1111/febs.15618
© 2020 Federation of European Biochemical Societies. This is the peer reviewed version of the following article: Eriksen, M.S., Nikolaienko, O., Hallin, E.I., Grødem, S., Bustad, H.J., Flydal, M.I., Merski, I., Hosokawa, T., Lascu, D., Akerkar, S., Cuéllar, J., Chambers, J.J., O’Connell, R., Muruganandam, G., Loris, R., Touma, C., Kanhema, T., Hayashi, Y., Stratton, M.M., Valpuesta, J.M., Kursula, P., Martinez, A. and Bramham, C.R. (2021), Arc self-association and formation of virus-like capsids are mediated by an N-terminal helical coil motif. FEBS J, 288: 2930-2955, which has been published in final form at https://doi.org/10.1111/febs.15618. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
https://rightsstatements.org/vocab/InC/1.0/
https://urn.fi/URN:NBN:fi-fe2021070140815
Tiivistelmä
Abstract
Activity-regulated cytoskeleton-associated protein (Arc) is a protein interaction hub with diverse roles in intracellular neuronal signaling, and important functions in neuronal synaptic plasticity, memory, and postnatal cortical development. Arc has homology to retroviral Gag protein and is capable of self-assembly into virus-like capsids implicated in the intercellular transfer of RNA. However, the molecular basis of Arc self-association and capsid formation is largely unknown. Here, we identified a 28-amino-acid stretch in the mammalian Arc N-terminal (NT) domain that is necessary and sufficient for self-association. Within this region, we identified a 7-residue oligomerization motif, critical for the formation of virus-like capsids. Purified wild-type Arc formed capsids as shown by transmission and cryo-electron microscopy, whereas mutant Arc with disruption of the oligomerization motif formed homogenous dimers. An atomic-resolution crystal structure of the oligomerization region peptide demonstrated an antiparallel coiled-coil interface, strongly supporting NT-NT domain interactions in Arc oligomerization. The NT coil–coil interaction was also validated in live neurons using fluorescence lifetime FRET imaging, and mutation of the oligomerization motif disrupted Arc-facilitated endocytosis. Furthermore, using single-molecule photobleaching, we show that Arc mRNA greatly enhances higher-order oligomerization in a manner dependent on the oligomerization motif. In conclusion, a helical coil in the Arc NT domain supports self-association above the dimer stage, mRNA-induced oligomerization, and formation of virus-like capsids.
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