Virus as renal functional genetic tools

Sharma, Abhishek; Cattavarayane, Sandhanakrishnan (2019-02-12)
 
Avaa tiedosto
Lataukset: 

URL:
https://doi.org/10.1007/978-1-4939-9021-4_12

Sharma, Abhishek
Cattavarayane, Sandhanakrishnan
Humana Press
12.02.2019

Sharma A., Cattavarayane S. (2019) Virus as Renal Functional Genetic Tools. In: Vainio S. (eds) Kidney Organogenesis. Methods in Molecular Biology, vol 1926. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9021-4_12

https://rightsstatements.org/vocab/InC/1.0/
© Springer Science+Business Media, LLC, part of Springer Nature 2019. This is a post-peer-review, pre-copyedit version of an article published in Methods in Molecular Biology. The final authenticated version is available online at https://doi.org/10.1007/978-1-4939-9021-4_12.
https://rightsstatements.org/vocab/InC/1.0/
doi:https://doi.org/10.1007/978-1-4939-9021-4_12
Näytä kaikki kuvailutiedot
Julkaisun pysyvä osoite on
https://urn.fi/URN:NBN:fi-fe202103258321
Tiivistelmä

Abstract

Viral vectors enable efficient transfection of ectopic DNA into hard to transfect cells. Viral vectors are normally used to obtain permanent modification of target cells, and tissues expect for the cases where integrase-deficient viruses are used. Here we describe a method to stably transfect metanephric mesenchyme cells isolated from the murine embryonic kidney at day E11.5. Using this method, it is possible to transfect hard to transfect cells and successfully evade host tissue immune response. Due to these advantages, this method has become one of the most frequently used in generating stable cell line, manipulation of tissues, and gene therapy.

Kokoelmat