Dual mode diffraction phase microscopy for quantitative functional assessment of biological cells
Talaikova, N.A.; Popov, A.P.; Kalyanov, A.L.; Ryabukho, V.P.; Meglinski, I.V. (2017-09-18)
Talaikova, N. A., Popov, A. P., Kalyanov, A. L., Ryabukho, V. P., & Meglinski, I. V. (2017). Dual mode diffraction phase microscopy for quantitative functional assessment of biological cells. Laser Physics Letters, 14(10), 105601. https://doi.org/10.1088/1612-202x/aa7d38
© 2017 Astro Ltd. This is an author-created, un-copyedited version of an article published in Laser Physics Letters. IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The Version of Record is available online at http://dx.doi.org/10.1088/1612-202X/aa7d38.
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https://urn.fi/URN:NBN:fi-fe2019050214019
Tiivistelmä
Abstract
A diffraction phase microscopy approach with a combined use of transmission and reflection imaging modes has been developed and applied for non-invasive quantitative assessment of the refractive index of red blood cells (RBCs). We present the theoretical background of signal formation for both imaging modes, accompanied by the results of experimental studies. We demonstrate that simultaneous use of the two modes has great potential for accurate assessment of the refractive index of biological cells, and we perform a reconstruction of spatial distribution of the refractive index of RBC in 3D.
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