Human tumor tissue-based 3D in vitro invasion assays

Åström, Pirjo; Heljasvaara, Ritva; Nyberg, Pia; Al-Samadi, Ahmed; Salo, Tuula (2018-01-10)
 
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https://doi.org/10.1007/978-1-4939-7595-2_19

Åström, Pirjo
Heljasvaara, Ritva
Nyberg, Pia
Al-Samadi, Ahmed
Salo, Tuula
Humana Press
10.01.2018

Åström P., Heljasvaara R., Nyberg P., Al-Samadi A., Salo T. (2018) Human Tumor Tissue-Based 3D In Vitro Invasion Assays. In: Cal S., Obaya A. (eds) Proteases and Cancer. Methods in Molecular Biology, vol 1731. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7595-2_19

https://rightsstatements.org/vocab/InC/1.0/
© Springer Science+Business Media, LLC 2018. This is a post-peer-review, pre-copyedit version of an book chapter published in Proteases and Cancer (ISBN: 978-1-4939-7594-5). The final authenticated version is available online at: https://doi.org/10.1007/978-1-4939-7595-2_19.
https://rightsstatements.org/vocab/InC/1.0/
doi:https://doi.org/10.1007/978-1-4939-7595-2_19
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https://urn.fi/URN:NBN:fi-fe2019102935473
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Abstract

Here we describe a protocol to utilize human benign leiomyoma tissue in in vitro 3D model that enables an assessment of cell invasion. The chapter also describes detailed instructions for image analysis to quantify the results. Leiomyoma is a benign tumor of the uterus which mimics authentic components of the tumor microenvironment including fibroblasts, vessels, collagen fibers, and extracellular protein composition. The leiomyoma invasion model represents a superior 3D model for cell invasion studies compared to the other non-human organotypic models.

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