The CapZ interacting protein Rcsd1 is required for cardiogenesis downstream of Wnt11a in Xenopus laevis
Hempel, Annemarie; Kühl, Susanne J.; Rothe, Melanie; Tata, Purushothama Rao; Sirbu, Ioan Ovidiu; Vainio, Seppo J.; Kühl, Michael (2017-02-22)
Annemarie Hempel, Susanne J. Kühl, Melanie Rothe, Purushothama Rao Tata, Ioan Ovidiu Sirbu, Seppo J. Vainio, Michael Kühl, The CapZ interacting protein Rcsd1 is required for cardiogenesis downstream of Wnt11a in Xenopus laevis, Developmental Biology, Volume 424, Issue 1, 1 April 2017, Pages 28-39, ISSN 0012-1606, https://doi.org/10.1016/j.ydbio.2017.02.014. (http://www.sciencedirect.com/science/article/pii/S001216061630865X)
© 2017 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/)
Wnt proteins are critical for embryonic cardiogenesis and cardiomyogenesis by regulating different intracellular signalling pathways. Whereas canonical Wnt/β-catenin signalling is required for mesoderm induction and proliferation of cardiac progenitor cells, β-catenin independent, non-canonical Wnt signalling regulates cardiac specification and terminal differentiation. Although the diverse cardiac malformations associated with the loss of non-canonical Wnt11 in mice such as outflow tract (OFT) defects, reduced ventricular trabeculation, myofibrillar disorganization and reduced cardiac marker gene expression are well described, the underlying molecular mechanisms are still not completely understood. Here we aimed to further characterize Wnt11 mediated signal transduction during vertebrate cardiogenesis. Using Xenopus as a model system, we show by loss of function and corresponding rescue experiments that the non-canonical Wnt signalling mediator Rcsd1 is required downstream of Wnt11 for ventricular trabeculation, terminal differentiation of cardiomyocytes and cardiac morphogenesis. We here place Rcsd1 downstream of Wnt11 during cardiac development thereby providing a novel mechanism for how non-canonical Wnt signalling regulates vertebrate cardiogenesis.
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