A purified MAA-based ELISA is a useful tool for determining anti-MAA antibody titer with high sensitivity
Shimomoto , Takasumi; Collins , Leonard B.; Yi, Xianwen; Holley, Darcy W.; Zhang , Zhenfa; Tian, Xu; Uchida , Koji; Wang , Chunguang; Hörkkö, Sohvi; Willis, Monte S.; Gold, Avram; Bultman , Scott J.; Nakamura, Jun (2017-02-21)
Shimomoto T, Collins LB, Yi X, Holley DW, Zhang Z, Tian X, et al. (2017) A purified MAA-based ELISA is a useful tool for determining anti-MAA antibody titer with high sensitivity. PLoS ONE 12(2): e0172172. https://doi.org/10.1371/journal.pone.0172172
© 2017 Shimomoto et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
https://creativecommons.org/licenses/by/4.0/
https://urn.fi/URN:NBN:fi-fe201704256251
Tiivistelmä
Abstract
Atherosclerosis is widely accepted to be a chronic inflammatory disease, and the immunological response to the accumulation of LDL is believed to play a critical role in the development of this disease. 1,4-Dihydropyridine-type MAA-adducted LDL has been implicated in atherosclerosis. Here, we have demonstrated that pure MAA-modified residues can be chemically conjugated to large proteins without by-product contamination. Using this pure antigen, we established a purified MAA-ELISA, with which a marked increase in anti-MAA antibody titer was determined at a very early stage of atherosclerosis in 3-month ApoE-/- mice fed with a normal diet. Our methods of Nε-MAA-L-lysine purification and purified antigen-based ELISA will be easily applicable for biomarker-based detection of early stage atherosclerosis in patients, as well as for the development of an adduct-specific Liquid Chromatography/Mass Spectrometry-based quantification of physiological and pathological levels of MAA.
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