Parallel <em>in vivo</em> monitoring of pH in gill capillaries and muscles of fishes using microencapsulated biomarkers
Borvinskaya, Ekaterina; Gurkov, Anton; Shchapova, Ekaterina; Baduev, Boris; Shatilina, Zhanna; Sadovoy, Anton; Meglinski, Igor; Timofeyev, Maxim (2017-05-15)
Ekaterina Borvinskaya, Anton Gurkov, Ekaterina Shchapova, Boris Baduev, Zhanna Shatilina, Anton Sadovoy, Igor Meglinski, Maxim Timofeyev. Parallel in vivo monitoring of pH in gill capillaries and muscles of fishes using microencapsulated biomarkers. Biology Open 2017 6: 673-677; doi: 10.1242/bio.024380
© 2017. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
Tracking physiological parameters in different organs within the same organism simultaneously and in real time can provide an outstanding representation of the organism’s physiological status. The state-of-the-art technique of using encapsulated fluorescent molecular probes (microencapsulated biomarkers) is a unique tool that can serve as a platform for the development of new methods to obtain in vivo physiological measurements and is applicable to a broad range of organisms. Here, we describe a novel technique to monitor the pH of blood inside the gill capillaries and interstitial fluid of muscles by using microencapsulated biomarkers in a zebrafish model. The functionality of the proposed technique is shown by the identification of acidification under anesthesia-induced coma and after death. The pH in muscles reacts to hypoxia faster than that in the gill bloodstream, which makes both parameters applicable as markers of either local or bodily reactions.
- Avoin saatavuus