Extracellular vesicles from oral squamous carcinoma cells display pro- and antiangiogenic properties
Dias Leite de Andrade, Ana Luiza; Ervolino de Oliveira, Carine; Rocha Dourado, Maurício; Carneiro Soares Macedo, Carolina; Vischi Winck, Flávia; Franco Paes Leme, Adriana; Salo, Tuula; Coletta, Ricardo D.; de Almeida Freitas, Roseana; Cavalcanti Galvão, Hébel (2017-09-08)
de Andrade ALDL, de Oliveira CE, Dourado MR, et al. Extracellular vesicles from oral squamous carcinoma cells display pro‐ and anti‐angiogenic properties. Oral Dis. 2018;24:725–731. https://doi.org/10.1111/odi.12765
This is the peer reviewed version of the following article: de Andrade, A. L. D. L., de Oliveira, C. E., Dourado, M. R., Macedo, C. C. S., Winck, F. V., Paes Leme, A. F., Salo, T., Coletta, R. D., de AlmeidaFreitas, R. and Galvão, H. C. (), Extracellular vesicles from oral squamous carcinoma cells display pro- and antiangiogenic properties. Oral Dis. Accepted Author Manuscript. doi:10.1111/odi.12765, which has been published in final form at http://dx.doi.org/10.1111/odi.12765. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
https://rightsstatements.org/vocab/InC/1.0/
https://urn.fi/URN:NBN:fi-fe2017113055178
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Abstract
Background: A new intercellular communication mode established by neoplastic cells and tumor microenvironment components is based on extracellular vesicles (EVs). However, the biological effects of the EVs released by tumor cells on angiogenesis are not completed understood. Here we aimed to understand the biological effects of EVs isolated from two cell lines of oral squamous cell carcinoma (OSCC) (SCC15 and HSC3) on endothelial cell tubulogenesis.
Methods: OSCC-derived EVs were isolated with a polymer-based precipitation method, quantified using nanoparticle tracking analysis and verified for EV markers by dot-blot. Functional assays were performed to assess the angiogenic potential of the OSCC-derived EVs.
Results: The results showed that EVs derived from both cell lines displayed typical spherical-shaped morphology and expressed the EV markers CD63 and Annexin II. Although the average particle concentration and size were quite similar, SCC15-derived EVs promoted a pronounced tubular formation associated with significant migration and apoptosis rates of the endothelial cells, whereas EVs derived from HSC3 cells inhibited significantly endothelial cell tubulogenesis and proliferation.
Conclusions: The findings of this study reveal that EVs derived from different OSCC cell lines by a polymer-based precipitation method promote pro- or antiangiogenic effects.
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