Postradiation matrix metalloproteinase-20 expression and its impact on dental micromorphology and radiation-related caries
Gomes-Silva, Wagner; Prado-Ribeiro, Ana Carolina; Brandão, Thais Bianca; Morais-Faria, Karina; de Castro Junior, Gilberto; Perez Mak, Milena; Ajudarte Lopes, Marcio; Marques Rocha, Marcelo; Salo, Tuula; Tjäderhane, Leo; de Goes, Mario Fernando (2017-03-31)
Gomes-Silva W, Prado-Ribeiro A, C, Brandão T, B, Morais-Faria K, de Castro Junior G, Mak M, P, Lopes M, A, Rocha M, M, Salo T, Tjäderhane L, de Goes M, F, Santos-Silva A, R, Postradiation Matrix Metalloproteinase-20 Expression and Its Impact on Dental Micromorphology and Radiation-Related Caries. Caries Res 2017;51:216-224
© 2017 S. Karger AG, Basel. This is the peer-reviewed but unedited manuscript version of the following article: Gomes-Silva W, Prado-Ribeiro A, C, Brandão T, B, Morais-Faria K, de Castro Junior G, Mak M, P, Lopes M, A, Rocha M, M, Salo T, Tjäderhane L, de Goes M, F, Santos-Silva A, R, Postradiation Matrix Metalloproteinase-20 Expression and Its Impact on Dental Micromorphology and Radiation-Related Caries. Caries Res 2017;51:216-224. The final, published version is available at http://www.karger.com/?doi=10.1159/000457806.
https://rightsstatements.org/vocab/InC/1.0/
https://urn.fi/URN:NBN:fi-fe2017120855521
Tiivistelmä
Abstract
Recent evidence suggests that head-and-neck radiotherapy (HNRT) increases active forms of matrix metalloproteinase-20 (MMP-20) in human tooth crowns, degrading the dentin-enamel junction (DEJ) and leading to enamel delamination, which is a pivotal step in the formation of radiation-related caries (RRC). Additional participation of enzymatic degradation of organic matrix components in caries progression was attributed to MMP-20 in dentin. Therefore, the current study tested the hypothesis that MMP-20 is overexpressed in the DEJ, dentin-pulp complex components, and carious dentin of post-HNRT patients, leading to detectable micromorphological changes to the enamel and dentin. Thirty-six teeth were studied, including 19 post-HNRT specimens and 17 nonirradiated controls. Optical light microscopy was used to investigate the micromorphological components of the DEJ, dentin-pulp complex components, and carious dentin. The samples were divided into 2 subgroups: nondemineralized ground sections (n = 20) and demineralized histological sections (n = 16). In addition, immunohistochemical analysis using the immunoperoxidase technique was conducted to semiquantitatively assess MMP-20 expression in the DEJ, dentin-pulp complex components, and carious dentin. No apparent damage to the DEJ microstructure or other dentin-pulp complex components was observed and no statistically significant differences were detected in MMP-20 expression (p > 0.05) between the irradiated and control groups. This study rejected the hypothesis that MMP-20 is overexpressed in the DEJ, dentin-pulp complex components, and carious dentin of post-HNRT patients, leading to detectable micromorphological changes. Hence, direct effects of radiation may not be regarded as an independent factor to explain aggressive clinical patterns of RRC.
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